Extraction d’ADN à partir de tissus
DNAdvance
Un kit de réactifs d’extraction d'ADN génomique (ADNg) haut débit, qui permet de purifier de l’ADN haute qualité à partir d’échantillons de tissus de mammifère.
- Compatible avec une variété d’outils d’analyse en aval (par ex., PCR, qPCR, génotypage SNP et NGS)
- Protocole manuel et adapté à l’automatisation
- Traitez trois plaques de 96 puits en ~1 heure grâce à l'automatisation.
Explore À partir de tissus Models
DNAdvance for Tissue Workflow
Caractéristiques
Combining the DNAdvance kit with Biomek Workstations offers a high throughput and walk-away solution; 15 mins hands-on-time for 96 samples and 1 hr 46 mins for 96 well plate.
DNAdvance consistently delivers superior recovery of nucleic acids. Isolate up to 40 ug of DNA from a variety of tissue types, e.g. brain, kidney, liver, lung, and tail.
DNA isolated with DNAdvance is compatible with a variety of downstream analysis tools such as PCR, qPCR, SNP genotyping, and sequencing.
Citations
Cain-Hom, C., Splinter, E., van Min, M., Simonis, M., van de Heijining, M., Martinez, M., . . . Warming, S. (2017, January 4). Efficient mapping of transgene integration sites and local structural changes in Cre transgenic mice using targeted locus amplification. Nucleic Acids Research, 45(8), e62. doi:10.1093/nar/gkw1329
- Used to extract DNA from mouse tissue
Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V., Nguyen, N. T., Zheng, Z., . . . Joung, J. K. (2015, July 23). Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature, 523(7561), 481–485. doi:10.1038/nature14592
- Used to extract DNA from U2OS.EGFP human cells
Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.
